Disease prevention

Reduced water for disease prevention

Dr. Sanetaka Shirahata Graduate of the Faculty of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

Reactive oxygen species (reactive oxygen species – ROS) have long been known to cause damage to bio-molecules and cell structures, which in turn lead to the development of various pathologies such as diabetes, cancer and ageing. Reduced water is defined as antioxidant water resulting from water reduction. Electrified low-hydrogen water (ERW) has been shown to be hydrogen-rich and can remove ROS in vitro (Shirahata et al., 1997). Reduction of the proton in water to active hydrogen (atomic hydrogen, hydrogen radical), which can rinse out ROS, is very easily caused by weak current compared to oxidation of the hydroxyl ion to the oxygen molecule. Activation of the water by magnetic field, collisions, minerals, etc. will also reduce the amount of water containing active hydrogen and/or hydrogen particles. Several natural waters, such as Hita Tenryosui water taken from deep underground in the city of Hita, Japan, Nordenau water in Germany and Tlacote water in Mexico are known for alleviating various diseases. We developed a sensitive method for detecting active hydrogen in reduced water and proved that not only ERW but also naturally reduced water described above contains active hydrogen and ROS in cultured cells. It is known that ROS reduces glucose absorption by inhibiting the insulin pathway in cultured cells. Reduced ROS in intravenous water and stimulated glucose uptake in the presence or absence of insulin in both L6 rat skeletal muscle cells and 3T3/L1 mouse adipocytes This insulin-like action of reduced water has been inhibited by robotinine, which is a specific inhibitor of PI-3 kinase, a key molecule in the insulin signal pathways. Reduced water resistance of insulin cells to diabetes and increased tolerance of type 2 diabetes to diabetes in model mellitus, suggesting that reduced amount of water can improve insulinindependent diabetes mellitus. Cancer cells are generally exposed to oxidative stress. Reduced amount of water results in impaired tumour phenotypes of human cancer cells, such as reduced growth rate, morphological changes, reduced ability to form colonies in soft agar, number-dependent shortening of telomeres, reduced ability to bind telomere-binding proteins and muffled metastases. Reduced amount of water inhibited growth of tumour cells transplanted to mice, showing their anticancer effect in vivo. Reduced water consumption will apply not only to medicine, but also to the food industry, agriculture and manufacturing.

Shirahata, S. et al: Electrolytically reduced water quantity absorbs active oxygen species and protects DNA from oxidative damage. Biochemistry. Biophyses. Resolution 234, 269174, 1997.