Bones

Influence of ionized alkaline water on bone tissue formation and maintenance

 

Rei Takahashi Zhenhua Zhang Yoshinori Itokawa (University of Kyoto, Faculty of Cancer Pathology and Biology, Fukui Preferred University)

The effect of ionized calcium-alkali water on the formation and maintenance of bone tissues in rats was investigated. In the absence of calcium in the diet no visible calcification was observed, but only the formation of osteoids was visible. Striking differences were found among groups administered a diet with 30% and 60% calcium. The lowest osteogenetic disturbances were observed in rats bred in ionized calcium water. Tibia and humor are more susceptible to calcium deficiency than femora. These results may indicate that calcium in drinking water effectively supplements osteogenesis in case of calcium deficiency in the diet. The mechanism of osteoid formation, such as the degree of calcium absorption from the intestine and the effect of ionized drinking water of alkaline calcium on bone structure maintenance in the aging process or in conditions of calcium deficiency, are studied.

Osteoporosis, which has recently attracted public attention, is defined as “bone brittleness conditions caused by a reduction in the number of bone frames and a deterioration in the bone microstructure”. One of the factors contributing to this problem is abnormal calcium metabolism, which in turn is caused by insufficient calcium uptake, a decrease in the rate of calcium absorption through the large intestine and an increase in the amount of calcium in the urinary tract. Under normal conditions, bones absorb old bones by regular metabolism through the formation of osteoids to maintain their strength and supporting function. It is becoming clear that bone remodeling at the tissue level is undergoing a process of activation, resorption, reversal, matrix synthesis and mineralisation. Another important function of bone is the storage of minerals, especially through coordination with the intestines and kidneys to control the calcium concentration in the blood. When something happens to this osteo metabolism, it results in abnormal morphological changes. In our analyses we focused mainly on bone changes to investigate the effect of calcium ionized alkaline ionized water on the osteo metabolism response system and its effectiveness. However, we have studied cannabis time in depth from a histological point of view. In other words, we compared the morphological and kinetic changes of osteogenesis with ionized alkaline water, tap water, and rat lactate.

Wistar’s three-week-old male rats were divided into 12 groups in terms of feeding conditions and drinking water. Feed was prepared with 0%, 30%, 60% and 100% of normal calcium and was administered freely. Three types of drinking water, tap water (urban water, about 6 ppm Ca), calcium lactate solution (Ca=40 ppm) and alkaline ionized water (Ca=40 ppm, pH=9, produced by Omco OMC Co., Ltd. NDX 4 LMC electrolyzer) were also given. The weight of rats, the amount of drinking water and feed as well as the Ca content of drinking water were determined daily. On the 19th and 25th day of the test, tetracycline hydrochloride was added to the feed for 48 hours to bring its concentration up to 30 mg/kg. On day 30, blood samples under nembuthal anaesthesia were taken, followed by tibia, humour and femur for the preparation of non-sticked samples. Their conditions of formation and rotation of osteoids were observed with the use of Villanueva bone stain and Villanueva goldner stain.

A comparison was made between three groups administered different types of drinking water and the same amount of Ca in the diet, in order to conclude that there was no significant difference in the rate of body weight gain and in the absorption of feed and drinking water. The alkaline group of ionized water had a significantly higher number of tibia and humor bones with higher concentrations of calcium in the bones.

In the group of 0% calcium, a drastic increase in the amount of osteoids in the feed was observed. There were no major differences in the type of drinking water. Almost no tetracycline was introduced to the tibia and humour, although a small amount was found in the pheromus. As a result, the osteogenesis went as far as the formation of osteoids, but it was likely that decalcification had not yet taken place or that most of the newly formed bones had been absorbed.

For the groups 30 % and 60 % of calcium in feed, the increase in the intake area of tetracycline was more pronounced, with greater clarity in descending order of ionised alkaline water, calcium lactate solution and tap water. Especially in the case of the tap water group, an uneven distribution between the tetracycline intake areas was observed. In the group of 100% calcium in feed, an improvement in osteogenesis was observed in the decreasing order of alkaline ionized water, calcium lactate solution and tap water. In any case, bone formation seemed to be in good condition at almost normal levels.

Alkaline ionized water was found to be effective in improving the osteogenesis of calcium deficiency in feed. The degree of dyssteogenesis also differed from region to region. This means that tibia and humeri are characterized by more significant dysosteogenesis than femora.

Moreover, there is a possibility that the osteo metabolism changes depending on the rate of calcium absorption through the intestine, regulation of renal outflow and functional thyroid control in the presence of alkaline ionized water. We are currently studying its effects on blood calcium levels. We are also investigating whether it is possible to stop bone spoilage by testing mice on rapidly aging models.